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HIV 1,2 Antibody Confirmation  

 
IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to NPHL@unmc.edu or call (402) 559-9444.

NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see NUlirt Guide).

Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing will not be performed without all required information.

Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.

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Test: HIV 1,2 Antibody Confirmation EIA  
Synonym: HIV confirmation, HIV Monospot 
Method: Rapid enzyme immunoassay  
Availability: Monday - Friday, within 72 hours of positive HIV Antigen/Antibody screen  
Specimen: Blood  
Collection Device: 6 ml SST (Gold) tube, 6 ml Clot (Red) tube, or 5 ml EDTA (Lavender) tube  
Volume: Minimum 0.5 ml serum or plasma  
Storage/Transport: Refrigerated. If transport of specimen not on the same day as collected, centrifuge and aliquot the serum or plasma into a clean tube.  
Unacceptable: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours  
Specimen Stability: Samples in sodium citrate, other body fluids, hemolyzed specimens, specimen on cells > 24 hours  
Reference Interval: Nonreactive  
Reportable Disease: Reactive is reportable 
Comments: Indeterminate results are reflex tested by the HIV RNA assay.

All HIV 2 reactive results will be confirmed by the CDC. 
Revised: 2/28/2017 
If you have questions about proper specimen collection, please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.

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