IMPORTANT! Pre-approval needed from state or local health department. Local or State Health Department. Send approval to
NPHL@unmc.edu or call (402) 559-9444.
NPHL has moved to an electronic ordering system, NUlirt, and it is now the preferred method to order all tests. Once ordered, print a "batch list" (see
NUlirt Guide).
Forms Required - Nulirt batch list or NPHL Test Request Form (completed in entirety) must accompany the specimen to include the following information: symptoms and date of onset, pregnancy status, travel and vaccine history. Testing
will not be performed without all required information.
Please call NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459 for further clarification.
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| Test: |
Hepatitis B Surface Antigen |
| Synonym: |
Australian Antigen; HAA; HBsAg; Hepatitis Associated Antigen |
| Method: |
Chemiluminescent Immunoassay |
| Availability: |
Daily; results in 24 hours |
| Specimen: |
Blood |
| Collection Device: |
6.0 mL SST (Gold) tube or
5.0 mL EDTA (Lavender) tube |
| Volume: |
2.0 mL serum or plasma (Min: 1.0 mL) |
| Storage/Transport: |
Refrigerate; if transport to lab is not same day as collected, aliquot the serum or plasma into a clean tube and label as "serum" or "plasma." |
| Unacceptable: |
|
| Specimen Stability: |
After separation from cells: Ambient, 8 hours; refrigerated, 14 days (if aliquoted to clean tube); frozen, 1 year (avoid multiple freeze/thaw cycles) |
| Reference Interval: |
Nonreactive |
| Reportable Disease: |
|
| Comments: |
Reactive samples will be confirmed by neutralization. Confirmatory testing is recommended on all reactive (positive) samples collected for prenatal screening. If patient fits this criteria, please call Regional Pathology Services to request confirmatory testing. |
| Revised: |
7/12/2011 |
If you have questions about proper specimen collection, please call
NPHL Client Services at (402) 559-2440 or toll free at (800) 334-0459.
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