| Test: | Vibrio Culture |
| Synonym: | Cholera culture |
| Method: | Standard reference procedures for Vibrio culture and identification |
| Availability: | Daily; final negative report at 2 days, positives called as soon as detected |
| Specimen: | Preserve stool in maintenance media (Cary-Blair or Enteric Plus) after collection |
| Collection Device: | Sterile stool container with maintenance media Specimen Labeling: Test subject to CLIA regulations and required 2 patient identifiers on specimen container and requisition forms |
| Volume: | 10.0 mL stool in maintenance media |
| Storage/Transport: | Prior to and during shipment: Ambient 15-25°C Shipping instructions, including specimen-handling requirements during transport: All screw-top tubes must be sealed with parafilm or tape. Package and ship as UN3373 Category B, Biological Substance. Place in biohazard bag with absorbent material. Transport by NPHL ground courier (NPHL brown shipper not required). If courier not available, contact Client Services (866) 290-1406 to arrange pickup. See instructions and shipping address: http://nphl.org |
| Unacceptable: | Nonsterile or leaking container, multiple specimens in 24 hour period, dry specimens, diapers, or delayed transport to lab in inappropriate preservative |
| Specimen Stability: | Ambient 15-25°C for 72 h |
| Reference Interval: | Culture negative for Vibrio spp |
| Reportable Disease: | Positive result should be immediately reported to local and state health department and isolates should be forwarded to the NPHL, refer to Nebraska DHHS Title 173, Communicable Diseases |
| Comments: | Pre-approval needed from state or local health department. Send documented approval to NPHL@unmc.edu or call (402) 559-9444. Indicate suspected organisms and available patient history, especially any recent travels, on the test request form. Maintenance media is available from Client Services. |
| Revised: | 7/11/2019 |